Structure-based design of a soluble human cytomegalovirus glycoprotein B antigen stabilized in a prefusion-like conformation
We thank members of the Texas Materials Institute EM facility (Karalee Jarvis, Raluca Gearba, and Xun Zhan) and The University of Texas at Austin Center for Biomedical Research Support (Michelle Mikesh) for technical assistance with negative-stain EM. We thank members of the Sauer Structural Biology Lab (Evan Schwartz, Axel Brilot) for technical assistance with cryo-EM data collection. We thank Kaci Erwin and James Guerra for technical assistance with mammalian cell culture, and we thank Justine Meccio for administrative support. We thank Emily J. Rundlet for providing helpful comments on the manuscript. Funding for these studies was provided by Dynavax Technologies Corporation. The Sauer Structural Biology Laboratory is supported by the University of Texas College of Natural Sciences and by award RR160023 from the Cancer Prevention and Research Institute of Texas.
Author contributions
M.R.S., P.O.B., A.G.L., A.R.R., J.A.G., R.S.M., L.Z., N.V.J., C.-L.H., K.P.K., J.D.C., S.R.P., J.A.M., D.Y., M.J.B., and J.S.M. designed research; M.R.S., P.O.B., A.G.L., A.R.R., J.A.G., R.S.M., L.Z., N.V.J., C.-L.H., M.C., K.P.K., C.M.C., and A.S.F. performed research; M.R.S., P.O.B., A.G.L., A.R.R., J.A.G., R.S.M., L.Z., N.V.J., C.-L.H., K.P.K., J.D.C., S.R.P., J.A.M., D.Y., M.J.B., and J.S.M. analyzed data; and M.R.S., P.O.B., A.G.L., J.A.M., and J.S.M. wrote the paper.
Competing interests
M.R.S., P.O.B., A.R.R., J.A.G., R.S.M., L.Z., N.V.J., C.-L.H., and J.S.M. are inventors on a patent application entitled “Prefusion-Stabilized CMV gB Proteins” (PCT/US2023/073369). J.D.C., D.Y., and M.J.B. are employees of Dynavax and hold Dynavax stock. The other authors declare that they have no competing interests.
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